Spectrophotometer, Nanovolume Measurement ⁠F3100

I. UV-Vis absorbance applications (Spectrophotometer)

Microvolume nucleic acid quantification: Accurately measures the concentration and assesses the purity of dsDNA, ssDNA and RNA samples (in pedestal mode, the dsDNA measuring range reaches up to 37,500 ng/μL without dilution).

Short oligonucleotide determination: Rapid quantification of Oligo DNA and Oligo RNA samples by entering a correction factor.

Micro Array application: Simultaneously determines the nucleic acid concentration and the concentration of up to two fluorescent dyes labeled on the sample (such as Cy3, Cy5) to calculate labeling efficiency.

Direct measurement of purified protein: Rapidly determines purified protein concentration by absorbance measurement at A280 and A205 without the need to build a standard curve.

Protein & Labels conjugate quantification: Analyzes protein content at 280 nm while measuring the absorbance of the conjugated fluorescent dye to assess the degree of labeling.

Protein quantification by colorimetric methods: Supports automatic standard curve generation to quantify impure protein or low-concentration samples using the following assays: Protein BCA, Bradford, Lowry, and Pierce 660.

Microbial cell density measurement (OD600): Monitors the growth of bacteria or cell populations in culture media by measuring absorbance at 600 nm.

Versatile UV-Vis spectrophotometry: Operates as a standard spectrophotometer, allowing spectral scanning and simultaneous measurement of up to 40 user-selected wavelengths across the full 185 nm to 910 nm range.

Absorbance kinetics: Sets up continuous measurement in a cuvette at defined time intervals (up to 5 stages) to monitor enzyme kinetics.

II. Fluorescence applications (Fluorometer)

High-sensitivity dsDNA quantification (Fluorometer dsDNA): A specialized measurement for NGS library quantification with an ultra-low limit of detection down to 5 pg/μL (0.005 ng/μL) with the dsDNA HS kit.

Fluorescent RNA quantification (Fluorometer RNA): Accurately determines RNA concentration, avoiding errors caused by degradation or interference from other impurities in the sample.

Fluorescent oligonucleotide quantification (Fluorometer Oligo): Selective quantification of single-stranded ssDNA fragments, PCR primers, or synthetic oligo samples.

Fluorescent protein quantification (Fluorometer Protein): Dedicated fluorescence assay to determine the concentration of highly dilute protein samples.

Direct fluorescence intensity measurement (Fluorescence): Directly measures the raw fluorescence intensity of the sample (without a standard curve), for rapid sample screening or research method development.

Fluorescence kinetics (Fluorometer Kinetics): Continuous or intermittent real-time reading of the sample's fluorescence signal to monitor the progress of biological reactions.